Enzymatic Assay of Nuclease S1

由 C Cappel 著作 · 2021 · 被引用 14 次 — Quantification and characterization of the 5' exonuclease activity of the lysosomal nuclease PLD3 by a novel cell-based assay · Authors · Affiliations.

It is essential that probes used in NPA analysis are all of a discrete length. High specific activity single-stranded RNA probes can be produced by in vitro ...

2011年4月6日 — Nuclease activity assay stained by EtBr · 1. 若以20% TBE gel分析，EtBr stain染膠30sec~1min，退染distain>20min · 2. anneal complementary pairs of ...

In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em = 304/369nm). The kit is simple, sensitive, high- ...

由 P Wyrzykowska 著作 · 2021 — Summary: The nuclease assay summarised in this protocol is a powerful and easy toolkit for analysis of the activity of enzymes connected to DNA/ ...

由 EC Sheppard 著作 · 2019 · 被引用 12 次 — Conventional nuclease assays predominantly involve the use of radioactive labelling to visualise DNA substrates on an agarose gel. The use of ...

The initial quality control assay for all components is an analysis for the presence of endo- and exonucleases. This involves incubating the test component with ...

To measure nuclease S1 activity, a spectrophotometric rate-determination assay is used at 260 nm. One unit of enzyme will cause 1 μg of single-stranded ...

由 M Fernández-Sierra 著作 · 2015 · 被引用 4 次 — In this work, we present fluorescence-based assays for real-time measurements of exonuclease and endonuclease kinetics. Our assays employ the oxazole yellow ...

由 U Sivagnanam 著作 · 2016 · 被引用 8 次 — Nuclease activity: gel assay. Nuclease assays were performed with 20 pmol of purified recombinant hPLSCR1, 200 ng of yeast/ human genomic DNA as ...